Publications & Abstracts
CLINICAL APPLICATION OF STANDARDIZED MICROFLUIDIC FLOW-BASED ASSAYS TO ASSESS ADHESIVE PROPERTIES OF SICKLE BLOOD
Dr. Patrick Hines chaired an education session on biomarkers and surrogate endpoints at the Foundation for Sickle Cell Disease Research (FSCDR) meeting the weekend of April 28-30th in Fort Lauderdale, FL. In addition to Dr. Hines, the session featured speakers from...read more
In this presentation to the Bioflux User Group in February 2017, Dr Hines describes our vision for clinical applications of a precise blood function assay, in particular to measure Thrombosis in pediatric ICU patients:...read more
Sevuparin Blocks Sickle Blood Cell Adhesion and Sickle-Leukocyte Rolling on Immobilized L-Selectin in a Dose Dependent Manner
Hemoglobinopathies, Excluding Thalassemia—Clinical Program: Oral and Poster Abstracts Session: 114. Hemoglobinopathies, Excluding Thalassemia—Clinical Poster II Sunday, December 4, 2016, 6:00 PM-8:00 PM Hall GH (San Diego Convention Center)...read more
Low molecular weight heparin inhibits sickle erythrocyte adhesion to VCAM-1 through VLA-4 blockade in a standardized microfluidic flow adhesion assay
British Journal of Hematology. Moira Lancelot, Jennell White, Sharada Sarnaik, Patrick Hines The vaso-occlusive events in sickle cell disease (SCD) begin in early childhood, warranting the need for more preventative and therapeutic interventions for those affected....read more
VLA-4 blockade by natalizumab inhibits sickle reticulocyte and leucocyte adhesion during simulated blood flow.
British Journal of Hematology. White JC, Krishnamoorthy S, Gupta D, Lancelot M, Moore N, Sarnaik S, Hobbs WE Abstract Very Late Antigen-4 (VLA-4, α4β1-integrin, ITGA4) orchestrates cell-cell and cell-endothelium adhesion. Given the proposed role of VLA-4 in sickle...read more
TR2/TR4 overexpression in a humanized sickle cell disease mouse model decreases RBC adhesion to VCAM-1.
Blood Cells Mol Dis. White JC, Pawar A, Fu G, Cui S, Tavernier F, Hamid M, Harro D, Giacherio D, Campbell AD, Hines PC. PMID: 26460253...read more
Increased erythrocyte adhesion to VCAM-1 during pulsatile flow: Application of a microfluidic flow adhesion bioassay.
Clin Hemorheol Microcirc White J, Lancelot M, Sarnaik S, Hines P. Abstract Sickle cell disease (SCD) is characterized by microvascular occlusion mediated by adhesive interactions of sickle erythrocytes (SSRBCs) to the endothelium. Most in vitro flow adhesion assays...read more
Pulsatile Flow Promotes Adhesion in Sickle Cell Disease: Clinical Application of a Microfluidic Bioassay.
Clinical Hemorheology and Microcirculation. White JC, Lancelot M, Sarnaik S, Hines P: Abstract Sickle cell disease (SCD) is characterized by microvascular occlusion mediated by adhesive interactions of sickle erythrocytes (SSRBCs) to the endothelium. Most in vitroflow...read more
International Society on Thrombosis and Haemostasis 2015 Congress – Toronto Nabil Othman2, Xiufeng Gao, MD2, Henry Walters, MD1,3, Patrick Hines, MD/PhD1,2 Children’s Hospital of Michigan, Detroit MI1, Wayne State University School of Medicine, Detroit, MI, USA,...read more
Evaluation of purified poloxamer-188 (vepoloxamer) on sickle red blood cell (RBC) adhesion and membrane fragility utilizing microfluidic-based flow adhesion bioassays
9th Annual Sickle Cell Disease Research and Educational Symposium (April 10 – 13, 2015) Conference Abstract Patrick C. Hines1, Jennell White1, Xiufeng Gao1, Ke Liu1, Marty Emanuele2 1Functional Fluidics, Detroit MI, 2Mast therapeutics, Inc. San Diego, CA Background...read more
Natalizumab Blocks VLA-4 Mediated Red Blood Cell Adhesion and Is a Potential Therapy for Sickle Cell Disease
Program: Oral and Poster Abstracts Type: Oral Session: 113. Hemoglobinopathies, Excluding Thalassemia – Basic and Translational Science: Innovative Therapeutic Approaches in Sickle Cell Disease Monday, December 8, 2014: 8:00 AM Yerba Buena Ballroom Salon 8 (San...read more
A novel role of h2-calponin in regulating whole blood thrombosis and platelet adhesion during physiologic flow.
Hines P, Gao X, White JC, D’Agostino A, Jin JP Abstract Calponin is an actin filament-associated protein reported in platelets, although the specific isoform expressed and functional role were not identified. The h2-calponin isoform is expressed in myeloid-derived...read more
Central Nervous System Events in Children with Sickle Cell Disease Presenting Acutely with Headache.
Journal of Pediatrics. Hines PC, McKnight T, Seto W, Kwiatkowski JL. Objective To determine the frequency of acute care visits and risk factors for central nervous system (CNS) events in children with homozygous sickle cell disease (SCD-SS) with an acute headache....read more
Pediatr Blood Cancer. Mazaheri, P, Nadkarni, G, Lowe, E, Hines, PC, Griffin, M, Resar, L. Abstract Ghosal hematodiaphyseal dysplasia syndrome (GHDD) is a rare clinical syndrome characterized by increased bone density and a severe, myelophthisic anemia. Few cases have...read more
Pulsed-dosing with oral sodium phenylbutyrate increases hemoglobin F in a patient with sickle cell anemia.
Pediatr Blood Cancer. Hines, PC, Dover, GJ, Resar, LMS. Abstract Increasing hemoglobin F (HbF) appears to be beneficial for patients with sickle cell anemia. We previously demonstrated that daily, oral sodium phenylbutyrate (OSPB) induces HbF synthesis in pediatric...read more
Novel Epinephrine and Cyclic AMP-Mediated Activation of B-CAM/Lu-Dependent Sickle (SS) RBC Adhesion.
Blood Hines PC, Zen Q, Burney SN, Shea DI, Ataga KA, Orringer EP, Telen MJ, Parise LV. Abstract The vasoocclusive crisis is the major clinical feature of sickle cell anemia, which is believed to be initiated or sustained by sickle (SS) red blood cell (RBC) adhesion to...read more
Blood 92 Lee SP, Cunningham ML, Hines PC, Joneckis CC, Orringer EP, Parise LV. Abstract Sickle red blood cell (RBC) adhesion to the endothelium and to exposed, underlying subendothelial proteins is believed to contribute to vascular occlusion in sickle cell disease....read more
Identification of a Region of Laminin That Adheres to Red Blood Cells of Sickle Cell Anemia Patients.
FAX: Student research journal at the UNC School of Medicine Hines PC, Parise LV.read more